Separation of plasmid-mediated extended spectrum β-lactamases by fast protein liquid chromatography (FPLC system)
نویسندگان
چکیده
منابع مشابه
Rapid purification of RNAs using fast performance liquid chromatography (FPLC).
We present here an improved RNA purification method using fast performance liquid chromatography (FPLC) size-exclusion chromatography in place of denaturing polyacrylamide gel electrophoresis (PAGE). The method allows preparation of milligram quantities of pure RNA in a single day. As RNA oligonucleotides behave differently from globular proteins in the size-exclusion column, we present standar...
متن کاملComparison of fast protein liquid chromatography (FPLC) with HPLC, electrophoresis & microcolumn chromatography techniques for the diagnosis of beta-thalassaemia.
BACKGROUND & OBJECTIVE beta-thalassaemia is a genetic disorder and an important health problem around the world. Quantitative haemoglobin A(2) (HbA(2)) levels are used for the diagnosis of beta-thalassaemia. The conventional methods are high performance liquid chromatography (HPLC), electrophoresis, and microcolumn chromatography techniques. We established a fast protein liquid chromatography (...
متن کاملExtended-Spectrum-Beta-Lactamases, AmpC Beta-Lactamases and Plasmid Mediated Quinolone Resistance in Klebsiella spp. from Companion Animals in Italy
We report the genetic characterization of 15 Klebsiella pneumoniae (KP) and 4 isolates of K. oxytoca (KO) from clinical cases in dogs and cats and showing extended-spectrum cephalosporin (ESC) resistance. Extended spectrum beta-lactamase (ESBL) and AmpC genes, plasmid-mediated quinolone resistance (PMQR) and co-resistances were investigated. Among KP isolates, ST101 clone was predominant (8/15,...
متن کاملExtended - spectrum Beta - lactamases
Beta-lactamase enzymes (BLs) have become the most worrisome mediators of antimicrobial resistance expressed by gram-negative bacteria (GNB). The multiple families of BLs have in common the ability to hydrolyze the -lactam rings of one or more members of the penicillins, cephalosporins, cephamycins, carbapenems, and monobactams. Extended-spectrum -lactamases (ESBLs) are a subset of BLs that conf...
متن کاملExtended-spectrum beta-lactamases.
At least 30 extended-spectrum beta-lactamases (ESB) have emerged responsible for resistance to indigestible beta-lactams (C3G) since their discovery in West Germany in 1983. Most of them are produced by enterobacteria and essentially K. pneumoniae which appeared susceptible to oxyimino-beta-lactams. A double-disk test was useful to detect such nosocomial isolates of enterobacteria (urines, bloo...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1990
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1990.tb04228.x